Chromatography lab report

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There are many forms of chromatography, but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases are important in this method; one that is stationary and one that is moving. Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two different phases between which are to be partitioned. The mixture is observed when it is in two different phase; a solid stationary phase and a liquid moving phase. A thin layer chromatograph plate is used to identify drug component under UV.
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Thin layer chromatography also known as TLC is the physical separation of a mixture into its individual components by distributing the components between a stationary phase the porous TLC plate and a mobile phase the solvent that moves through the stationary phase and carries the material that needs to be separated. The driving force to separate components is capillary action. This method can be used to determine how many different components usually non-volatile are in a sample. Column chromatography is a method to physically separate all of the components also usually non-volatile of a mixture. The driving force to separate components is gravity. Both of these methods work based on polarity differences between components in a sample. In order to perform thin layer chromatography, seven porous plates needed to be obtained that were in good condition with no chips or cracks.
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The aim of this test was to split up and isolate the various photosynthetic pigments, entirely on spinach leaves and draw out them using the newspaper chromatography method. Desire to was also to determine the relative levels of chlorophyll a and chlorophyll b using their absorption spectra. Leaves of fresh spinach were used to separate its photosynthetic pigments and then measure their absorption and percentage between chlorophyll a and chlorophyll b. Apart from the paper chromatography method, another method called spectrophotometry was found in order to gauge the absorption of light of each pigment. The retention Rf value for every one of the pigments and the focus of chlorophyll a and b were calculated, and every one of the results were put in tables.
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This experiment also allows monitoring reactions of organic molecules, and determines the identity of a mixture of compounds. Hexane1Molecular Molarity: Page Limit: report must. Acetaminophen C8H9NO2 2.
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