Cryo-electron microscopy cryo-EM has rapidly expanded with the introduction of direct electron detectors, improved image-processing software and automated image acquisition. Its recent adoption by industry, particularly in structure-based drug design, creates new requirements in terms of reliability, reproducibility and throughput. This paper will cover the lessons learned during the setting up of these facilities, including two Consortium Krios microscopes and preparation laboratories, several Glacios microscopes at Consortium member sites, and a Krios and Glacios at eBIC for Industry, regarding site evaluation and selection for high-resolution cryo-EM microscopes, the installation process, scheduling, the operation and maintenance of the microscopes and preparation laboratories, and image processing. Keywords: cryo-EM ; facility setup ; facility management ; industry. Transmission electron microscopy TEM laboratory designs and cryo-EM facility management play a crucial role in the optimal operation of the microscopes and their output O'Keefe et al. However, laboratory-design reports are focused largely on materials science and facility management on academic cryo-EM.
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Oklahoma City Community College
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eCall – The EU emergency-call system
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Socially-assistive robots SAR have been used to reduce pain and distress in children in medical settings. Patients who perceive empathic treatment have increased satisfaction and improved outcomes. We sought to determine if an empathic SAR could be developed and used to decrease pain and fear associated with peripheral IV placement in children.